The ECDC Communicable Disease Threats Report (CDTR) is a weekly bulletin for epidemiologists and health professionals on active public health threats. This issue of the ECDC Communicable Disease Threats Report (CDTR) covers the period 15 – 21 March 2025 and includes updates on cholera, Middle East respiratory syndrome, an overview of respiratory virus epidemiology in the EU/EEA, avian influenza, chikungunya, Ebola, and the risk of severe infections, carriage and cross-border transfer of carbapenem-resistant bacteria in victims of the fire at Pulse nightclub in North Macedonia.